Imec and Sarcura introduce scalable on-chip detection of human white blood cells

On chip flow cytometer
On chop flow cytometer. Photo: Sarcura/imec

Sarcura GmbH, an Austrian early-stage technology start-up, and imec, a research and innovation hub in nanoelectronics and digital technologies, have presented a proof-of-concept on-chip flow cytometer using integrated photonics.

Published in Scientific Reports, the innovation offers a platform for the detection and discrimination of human leukocytes and is a stride towards cost-effective, scalable, and highly parallelized cell analysis.

Left: Schematic cross-section of the chip layer stack, indicating light coupling into the chip, cell illumination, and collection and detection of cell scattering signals. Right: Experimental scatter plot of a full peripheral blood mononuclear sample measured with the on-chip flow cytometer.

Accurate identification of human cells is a key operation in modern medicine, important for understanding disease mechanisms and advancing targeted and personalized treatments. With the advent of cell manufacturing, living cells can now be engineered to function as treatments, notably in groundbreaking therapies like CAR-T immune cell therapy for cancer. The ability to identify these therapeutic cells in complex cell products at high throughput is crucial, and often time sensitiThe method of choice today is flow cytometry, which enables characterization of cell populations based on the physical and chemical characteristics of individual cells as they flow past a laser. However, the current implementation involves bulky instrumentation, complex and manual workflows (posing contamination risks), and high operational costs. These challenges hinder widespread availability and adoption of cell therapies in decentralized settings.

Imec and Sarcura‚Äôs on-chip flow cytometer was fabricated on imec’s 200mm CMOS pilot line. The opto-fluidic chip features a material stack facilitating both cell illumination and capturing of scattered light through waveguide optics, and precise cell delivery to the detection points using microfluidic channels.

“Silicon photonics, as successfully demonstrated in this novel photonic chip, is the revolutionary and essential building block that merges single-cell detection capabilities with massive parallelization on a dramatically miniaturized footprint. This breakthrough opens new possibilities for addressing previously unsolved challenges in applications such as cell therapy manufacturing,” said Daniela Buchmayr, Sarcura CEO and co-founder.

Niels Verellen, scientific director at imec, noted: “We have demonstrated, for the first time, that a monolithically integrated biophotonic chip can be used to collect optical scattering signals that allow the discrimination of lymphocytes and monocytes from a patient’s blood sample, rivaling the performance of commercial cytometers. The main advantage lies in the potential for dense parallelization of multiple flow channels to boost the system throughput.”

In the next phase, the compact, alignment-free design should enable billions of cells to be identified within a limited amount of time. The chip architecture seamlessly integrates with imec’s previously developed bubble jet cell sorting module, compatible with wafer-scale fabrication. Also, the photonic components and layout can be tailored to suit specific applications. The companies said the proof-of-concept is a leap towards cost-effective, scalable, and highly parallelized cell sorting platforms.

Jim Cornall is editor of Deeptech Digest and publisher at Ayr Coastal Media. He is an award-winning writer, editor, photographer, broadcaster, designer and author. Contact Jim here.